Protein transfer from the gel to membrane is the most variable part of a Western blot. Even if you know the optimal buffer and transfer conditions, there are always bubbles, uneven protein transfer or other transfer artefacts, which you don’t ...
Posts Categorized: Western blots
Setting up a new Western blot? Thanks to increased assay sensitivities, readily available antibody-coupling products and improved conjugate reagents, you have a big decision to make: Should you do a direct or an indirect Western blot? The ...
Ordinarily we don't like to recommend stripping and reprobing a Western blot. A traditional stripping protocol results in uneven removal of proteins leading to loss of genuine signal, high background and unquantifiable data. But we've changed ...
New to fluorescence imaging systems? Or maybe you are considering buying a new one? Don't you wish there was an easy way to evaluate the performance of a fluorescent imager (other than wasting your time running your own Western blot)? Well ...