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The Electric Field The transfer of proteins from gel to membrane is accomplished via an electric field. This field is dependent on Ohm’s law, which relates voltage (V), current (I), and resistance (R) as V = I × R. The applied voltage and ...

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To dry, or not to dry: that is the question. After a long day preparing your fluorescent Western blot, it is tempting to rush to imaging so that you can see the fruits of your labor. There’s nothing wrong with this work-flow but you will likely ...

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In order to generate high quality Western blot data, it is imperative to perform protein normalization. The goal of normalization is to minimize the impact of experimental errors, which include inconsistent sample preparation, unequal sample loading ...

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We often get this question. First off, you shouldn’t feel that you have to choose one method all of the time. Advansta offers all of the reagents you need for both chemiluminescent and fluorescent Westerns to allow for a flexible workflow. If you ...

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