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Electrophoresis

Blotting sponge pads
Blotting sponge pads

Transfer sponge pads for more reproducible Western blot transfers

  • Resist compression - maintain proper pressure in transfer sandwiches without adding extra blotting papers
  • Retain thickness - can be reused over multiple experiments
  • Convenient - two sizes (8 x 11 cm and 9 x 15 cm) compatible with a variety of transfer apparatuses
  • Standardized - uniform flat construction ensures reproducible and consistent transfers
Transfer membranes
Transfer membranes

Convenient nitrocellulose and PVDF membranes for Western blotting

  • Performance – quality controlled for background-free Western blots
  • Convenience – membranes available pre-cut and as rolls
  • High signal-to-noise ratio – low background signal for clearer results
  • Flexibility – choose the right membrane for your Western blotting application

 

Membrane Options:

WesternBright PVDF-FL: fluorescent detection
WesternBright PVDF-CL: chemiluminescent detection; best stability for stripping and re-probing chemiluminescent blots
PVDF membranes available as rolls or pre-cut (7x9 cm, 10x15 cm, or 13x18 cm)

WesternBright NC: all-purpose nitrocellulose membrane
Nitrocellulose membranes available as rolls or pre-cut (8x10 cm)

Visio
Visio

Sample-loading buffer and protein stain in one

  • Instant results – see protein bands immediately as electrophoresis proceeds
  • Sensitive – detect as little as 10 to 25 ng per band
  • Fast protocol – no different from standard electrophoresis protocols - replace your current loading dye with Visio and samples labeled and ready to load after heating for 10 minutes
  • Flexible – use with reducing and non-reducing gels
  • Compatible – can be used with downstream mass spectrometry

 

Protein sample loading buffers
Protein sample loading buffers

Pre-mixed standard Laemmli loading buffers for protein electrophoresis

  • Pre-mixed - just add an equal volume to your protein sample (supplied as 2x)
  • Obtain reproducible results with standardized buffers
  • Choose from reducing or non-reducing loading buffers
FLASHBlot transfer buffer
FLASHBlot transfer buffer

Ready-to-use transfer buffer
Designed for faster, more efficient protein transfer with increased sensitivity

FLASHBlot is not your average transfer buffer.

  • Faster than traditional buffers: better results in 20 minutes
  • More sensitive detection for low abundance proteins
  • Better detection of post-translationally modified proteins
  • Better transfer of ALL proteins, including high molecular weight proteins
  • Conveniently suitable for any wet transfer set-up

 

“ We were pleasantly surprised to see that with FLASHBlot we observed the same transferring levels at 25 min than with the lengthier transfer protocol… including for the 300 kDa protein. This reagent will shorten considerably our experimental time. ”  - Assistant Professor, UCSF

 

Afyon SDS-PAGE sample preparation kit
Afyon SDS-PAGE sample preparation kit

Kit for fast, easy concentration and purification of samples for SDS-PAGE

 

  • Purify - Remove contaminants that can interfere with electrophoresis (GuHCl, urea, Ammonium sulfate, etc)
  • Quick - Protein samples ready to load in less than 10 minutes
  • Safe - and non-toxic - no DMSO required
  • Compatible with SDS-PAGE and downstream Western blotting
  • Many times faster than concentration and buffer exchange via ultrafiltration or dialysis

 

AdvanStain Scarlet
AdvanStain Scarlet

Fluorescent protein stain for gels and blots

 

  • Sensitive – detect less than a nanogram of protein per spot
  • Convenient – simple 3-hour protocol
  • Flexible – stain gels or membranes
  • Reversible – destain for downstream Western blotting or mass spectrometry
  • No speckling – clean background for better data
  • Safe – biodegradable and contains no heavy metals for increased safety and simple disposal
  • Compatible – image with any laser or CCD imaging system. Compatible excitation wavelengths include green (543, 532 nm), blue (488 nm), violet (405 nm) and UV (302, 365 nm). Maximum emission wavelength is 610 nm

 

Includes: AdvanStain Scarlet Dye Concentrate
Powder A, for preparation of Fix Solution
Powder B, for preparation of Stain Buffer

 

Avant buffer pouches
Avant buffer pouches

Convenient, pure, fast buffer preparation

 

  • Quality – molecular biology-grade buffers of highest purity
  • Convenient – pre-measured powder in sealed pouches
  • Fast – dissolve and use right away
  • Standardized – guaranteed reproducible results for your electrophoresis gels and Western blots