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Immunoprecipitation (IP) is a method used to enrich or purify your target protein from a complex mixture, such as a cell lysate, using a solid support. After IP you can analyze your samples by various techniques such as Western blot, ELISA or Mass ...

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Loading control antibodies are a critical Western blotting tool that is often overlooked. They play an important role, ensuring that that you have loaded and transferred equivalent amounts of protein across your Western blot. If you are planning on ...

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Selecting a primary antibody will be one of the most important decisions that you will make when designing your next experiment. Choose wisely because an antibody with poor specificity will wreak havoc on your data, making interpretation nearly ...

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Everyone knows that the best offense is a good defense. So how do you defend your Western blot against background and non-specific binding? The best strategy ensures that your membrane is sufficiently blocked and your target’s epitopes are not ...

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