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Protein transfer from the gel to membrane is the most variable part of a Western blot. Even if you know the optimal buffer and transfer conditions, there are always bubbles, uneven protein transfer or other transfer artefacts, which you don’t ...

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Ordinarily we don't like to recommend stripping and reprobing a Western blot.  A traditional stripping protocol results in uneven removal of proteins leading to loss of genuine signal, high background and unquantifiable data. But we've changed ...

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New to fluorescence imaging systems?  Or maybe you are considering buying a new one?  Don't you wish there was an easy way to evaluate the performance of a fluorescent imager (other than wasting your time running your own Western blot)? Well ...

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