Protein transfer from the gel to membrane is the most variable part of a Western blot. Even if you know the optimal buffer and transfer conditions, there are always bubbles, uneven protein transfer or other transfer artefacts, which you don’t ...
Posts Categorized: Advansta products
Ever thought about using directly-labeled primary antibodies for your assay? You can buy them commercially, or you can label them yourself. With new kits and technology, it's fairly easy to do. Here are some reasons why you might want to add ...
Ordinarily we don't like to recommend stripping and reprobing a Western blot. A traditional stripping protocol results in uneven removal of proteins leading to loss of genuine signal, high background and unquantifiable data. But we've changed ...
New to fluorescence imaging systems? Or maybe you are considering buying a new one? Don't you wish there was an easy way to evaluate the performance of a fluorescent imager (other than wasting your time running your own Western blot)? Well ...