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The first step to creating publication worthy Western blots Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis, phew that was a mouthful! Let’s go with SDS-PAGE for short. This technique is used to separate proteins in a complex sample ...

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Increasing Western Blot Data Output The Western Blot is one of the first and most commonly utilized tools that researchers go to during the discovery process. However, once your target has been confirmed and your antibodies validated there is very ...

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The Electric Field The transfer of proteins from gel to membrane is accomplished via an electric field. This field is dependent on Ohm’s law, which relates voltage (V), current (I), and resistance (R) as V = I × R. The applied voltage and ...

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To dry, or not to dry: that is the question. After a long day preparing your fluorescent Western blot, it is tempting to rush to imaging so that you can see the fruits of your labor. There’s nothing wrong with this work-flow but you will likely ...

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