Sample preparation

Protein sample loading buffers
Protein sample loading buffers

Pre-mixed standard Laemmli loading buffers for protein electrophoresis

  • Pre-mixed - just add an equal volume to your protein sample (supplied as 2x)
  • Obtain reproducible results with standardized buffers
  • Choose from reducing or non-reducing loading buffers
Afyon SDS-PAGE sample preparation kit
Afyon SDS-PAGE sample preparation kit

Kit for fast, easy concentration and purification of samples for SDS-PAGE

 

  • Purify - Remove contaminants that can interfere with electrophoresis (GuHCl, urea, Ammonium sulfate, etc)
  • Quick - Protein samples ready to load in less than 10 minutes
  • Safe - and non-toxic - no DMSO required
  • Compatible with SDS-PAGE and downstream Western blotting
  • Many times faster than concentration and buffer exchange via ultrafiltration or dialysis

 

G-25 Desalting Spin Columns
G-25 Desalting Spin Columns

Rapid buffer exchange for protein samples

 

  • Fast protocol – buffer exchange or salt removal complete in less than 10 minutes
  • Convenient – pre-made single-use columns
  • Performance – high desalting capacity
  • Versatile – may be used for multiple applications including desalting, buffer exchange, and removal of free dye or any compound with molecular weight below 5000