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We’ve all been there before…. You worked hard all day and think that you have executed a brilliant experiment, only to realize that you generated data that looks like a hot mess. This is the worst feeling ever! The worst part is that there is ...

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Have you ever wondered why there are so many protein quantitation assays to choose from? I think I ask myself this question every time I need to use one! Accurate determination of protein concentration is critical for many experiments. ...

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ELISA Technical Tips Enzyme-linked immunosorbent assay (ELISA or EIA) is a plate-based assay used to measure the concentration of an analyte in a sample. The analyte may be a peptide, protein, antibody or hormone. Depending on the approach you ...

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Let’s say you have worked very hard and you have developed a perfect ELISA assay. Your plate is coated to perfection, non-specific binding is blocked and all samples have been properly diluted to fit your assay’s limits. Don’t let the wrong ...

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