ELISA Technical Tips Enzyme-linked immunosorbent assay (ELISA or EIA) is a plate-based assay used to measure the concentration of an analyte in a sample. The analyte may be a peptide, protein, antibody or hormone. Depending on the approach you ...

Let’s say you have worked very hard and you have developed a perfect ELISA assay. Your plate is coated to perfection, non-specific binding is blocked and all samples have been properly diluted to fit your assay’s limits. Don’t let the wrong ...