Back in the 1970’s, Edwin Southern came up with an ingenious method to study DNA fragments. By integrating three key innovations - restriction enzymes, gel electrophoresis and blotting techniques he greatly increased the speed and simplicity of ...
Posts Categorized: phosphorylation
So you think your protein might be phosphorylated? Maybe it runs as a fuzzy smear on the gel, slightly higher than predicted. Or perhaps you think it is involved in a signaling cascade. In any case, if you suspect phosphorylation, then you ...
Detecting phosphorylated proteins on a Western blot isn't really different from doing a regular Western blot. But there are a few tricks that can help you get the perfect blot: ...
Some studies suggest that up to 30% of proteins are phosphorylated in eukaryotic cells. Whoa. So chances are, if you study proteins, you are going to be examining protein phosphorylation at some point in your career. But how do you know which ...