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Last week I took some time to reflect on how all the fine details of technique can impact the accuracy of my Western blots. These are all the little things that have become second nature to me over the years. After running thousands of Western ...

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There’s nothing more frustrating than ending your day with too many bands on your Western blot. Was it my sample? My blocker? My antibodies? If you are working with a new sample or antibody the answer is probably, I have no clue. Cue the painful ...

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Whether you are working with cells or tissues, protein extraction can be a harsh process that damages your target. Your options are to either physically rip your cells apart or burst them open with chemicals. No pressure, but if you mess this part ...

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