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Since its inception in 1979, the Western blotting process has remained relatively unchanged. It was and continues to be a deceptively technically challenging method. When it works well, it is a fabulous tool. When it doesn’t work, the researcher ...

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The first step to creating publication worthy Western blots Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis, phew that was a mouthful! Let’s go with SDS-PAGE for short. This technique is used to separate proteins in a complex sample ...

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Increasing Western Blot Data Output The Western Blot is one of the first and most commonly utilized tools that researchers go to during the discovery process. However, once your target has been confirmed and your antibodies validated there is very ...

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The Electric Field The transfer of proteins from gel to membrane is accomplished via an electric field. This field is dependent on Ohm’s law, which relates voltage (V), current (I), and resistance (R) as V = I × R. The applied voltage and ...

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