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Everyone knows that the best offense is a good defense. So how do you defend your Western blot against background and non-specific binding? The best strategy ensures that your membrane is sufficiently blocked and your target’s epitopes are not ...

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Prepare yourself for a true story. After graduation, a newly minted postdoc left our molecular biology lab to work in a clinical science lab in Germany. He developed a reputation in our lab for his SDS-PAGE gels, which gave the sharpest bands on ...

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Sometimes your Western blot bands are not straight but  “smile” - the bands look more like crescent moons than straight lines. And, unlike your smile, a smiling Western is not a good thing. Band distortion makes it harder to determine the ...

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