Back in the 1970’s, Edwin Southern came up with an ingenious method to study DNA fragments. By integrating three key innovations - restriction enzymes, gel electrophoresis and blotting techniques he greatly increased the speed and simplicity of ...

Before you start your next Western, we can help you decide which membrane will be best for your experiment. The quick blot Start with a quick experiment that doesn't require the highest level of sensitivity. In this case, nitrocellulose is more ...

When did you last change your foam inserts in the blotting apparatus, the blotting sponge pads? The answer is probably “never”. Because if the transfer works, why bother? I worked in several labs and in all of them, we hardly ever changed the ...

I love using fluorescent antibodies instead of the old-fashioned (well, I think they are) HRP-conjugated ones for Western Blots. If you work with dye-conjugated antibodies, you don't stress over guessing the right exposure time, while your time’s ...