Fluorescent Western blotting

WesternBright MCF
WesternBright MCF

Multi-color fluorescent Western blotting in one kit

Try multicolor fluorescent western blotting today. Our kit includes everything except primary antibodies.

  • Multiplexing – visualize two proteins simultaneously!
  • Sensitivity –10x greater sensitivity than Cy dyes; pictogram detection
  • Quality Reagents – includes AdvanBlock™-PF blocking and AdvanWash /AdvanWash-IR for washing
  • Fast results – 3.5 hours for entire protocol
  • Compatible with imaging systems that detect Cy3 and Cy5 (MCF) or near-IR imaging systems (MCF-IR)
  • Fluorescent Detection – choose between visible (APC and RPE) or near IR (IR700 and IR800) kits with SpectraDye™ secondary antibodies

 

Kit Includes:
Secondary antibodies
Blocking solution
Washing solution
Pre-cut low-autofluorescence PVDF membranes
Background quenching sheets

 

SpectraDye Secondary Antibodies
SpectraDye Secondary Antibodies

Fluorescently labeled secondary antibodies

  • Fluorescent detection –secondary antibodies with a range of fluorescent conjugates
  • Performance – strong signal and no cross-reactivity
  • Convenience – each antibody demonstrated to provide optimal results with Advansta's Western blotting systems
  • Flexibility – choose from visible or near IR fluorescent dyes and target species best suited to your application (see product listings)

2nd AB excitation emission chart

 

SpectraDye Antibody Labeling Kits
SpectraDye Antibody Labeling Kits

Make your own fluorescent antibody in one easy step.

  • Quick and easy – one-step labeling in 30 minutes
  • Versatile - Labeled antibodies are compatible with immunofluorescence microscopy, flow cytometry, Western blotting
  • Flexible - choose from 6 commonly used fluorescent dyes
  • Save time and money – no secondary or extra steps, once you label your primary
  • Save antibody – labeling reaction requires as little as 10 µg antibody

 

Kit includes:
Antibody Labeling Buffer
SpectraDye Dye Solution*
Quenching Solution
Neutralization Buffer

Sufficient reagents to label up to 1 mg of antibody
*Except kit K-11060-010

 

Spectradyes

Fluorescent streptavidin conjugates
Fluorescent streptavidin conjugates

Fluorescent streptavidin conjugates to detect biotinylated proteins

  • For Western blotting, protein arrays, flow cytometry, and more
  • Extremely bright fluorescence for high sensitivity
  • Compatible with imaging systems that detect Cy3 and Cy5 or similar dyes
  • Red or green fluorescent conjugates to choose from
Background Quenching Sheets
Background Quenching Sheets

An added layer to decrease background
For improved imaging of blots and gels

  • Better images by removing background fluorescence and stray light
  • Improved signal to noise ratios
  • Simple – just place sheet under the blot or gel
  • Compatible with chemiluminescent and fluorescent Western blots
  • Compatible with DNA gels and protein stains - including SYBR green, SYBR Gold, ethidium bromide, SYPRO® Orange, SYPRO Ruby and more

 

Transfer membranes
Transfer membranes

Convenient nitrocellulose and PVDF membranes for Western blotting

  • Performance – quality controlled for background-free Western blots
  • Convenience – membranes available pre-cut and as rolls
  • High signal-to-noise ratio – low background signal for clearer results
  • Flexibility – choose the right membrane for your Western blotting application

 

Membrane Options:

WesternBright PVDF-FL: fluorescent detection
WesternBright PVDF-CL: chemiluminescent detection; best stability for stripping and re-probing chemiluminescent blots
PVDF membranes available as rolls or pre-cut (7x9 cm, 10x15 cm, or 13x18 cm)

WesternBright NC: all-purpose nitrocellulose membrane
Nitrocellulose membranes available as rolls or pre-cut (8x10 cm)

FLASHBlot transfer buffer
FLASHBlot transfer buffer

Ready-to-use transfer buffer
Designed for faster, more efficient protein transfer with increased sensitivity

FLASHBlot is not your average transfer buffer.

  • Faster than traditional buffers: better results in 20 minutes
  • More sensitive detection for low abundance proteins
  • Better detection of post-translationally modified proteins
  • Better transfer of ALL proteins, including high molecular weight proteins
  • Conveniently suitable for any wet transfer set-up

 

“ We were pleasantly surprised to see that with FLASHBlot we observed the same transferring levels at 25 min than with the lengthier transfer protocol… including for the 300 kDa protein. This reagent will shorten considerably our experimental time. ”  - Assistant Professor, UCSF

 

Development folders
Development folders

Transparent plastic supports for handling and imaging blots

  • Clear images – avoid the wrinkling or bunching that can happen with plastic wrap
  • Suitable for all blots – development folders are suitable for chemiluminescence and fluorescence imaging
  • Safe blot handling – move your blot without scratching or putting pressure on the surface
  • Convenient size – image up to 2 blots simultaneously in one folder
Incubation trays
Incubation trays

Convenient containers for staining and washing gels and membranes

  • Smooth interior – protects membranes and gels from scratches
  • Convenient – multiple sizes (5x7, 6x9, 9x11,10.5x15.5 cm) and designs available for different gels and blots
  • Attached lid – to protect experiments from dust or debris that can cause speckles on blot images
  • Save – minimize antibody and buffer usage by using appropriately sized containers
  • Multi-chamber trays – process 6 mini-blots simultaneously, each chamber requires as little as 3 mL of solution

 

AdvanBlock-PF blocking solution
AdvanBlock-PF blocking solution

Non-protein blocking solution for fluorescent and chemiluminescent Western blotting

  • Low background - Protein-free block buffer for lower background with certain antibodies
  • Optimized - Stabilizes fluorescence of WesternBright MCF fluorescent secondary antibodies
  • Versatile - Compatible with fluorescent and chemiluminescent detection
AdvanBlock-Chemi blocking solution
AdvanBlock-Chemi blocking solution

Signal-enhancing Western blot block solution 
for the best chemiluminescent signals

  • Enhanced signal – greatly enhances Western blotting signals
  • Decreased background – decreased non-specific bands and lowered background
  • Sensitivity – get better signals for low protein sample concentrations
  • Convenience – Pre-made buffer means you save preparation and optimization time

AdvanBlock-EIA blocking solution
AdvanBlock-EIA blocking solution

Blocking solution for ELISAs and immunoassay plates

  • Decreases background
  • Decreases well-to-well variability
  • Decreases non-specific binding
  • Ready-to-use solution