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Fluorescence done right is a great way to multiplex and generate data for quantitative analysis. Since it address some of the limitations faced with chemiluminescence detection, it makes perfect sense why fluorescent Western blotting continues to ...

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When it comes time to transfer your proteins, attention to detail is important as this is one of the most crucial steps for a successful Western blot. One essential element that can determine the efficiency of your transfer is the transfer buffer. ...

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Since its inception in 1979, the Western blotting process has remained relatively unchanged. It was and continues to be a deceptively technically challenging method. When it works well, it is a fabulous tool. When it doesn’t work, the researcher ...

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The first step to creating publication worthy Western blots Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis, phew that was a mouthful! Let’s go with SDS-PAGE for short. This technique is used to separate proteins in a complex sample ...

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