I love using fluorescent antibodies instead of the old-fashioned (well, I think they are) HRP-conjugated ones for Western Blots. If you work with dye-conjugated antibodies, you don't stress over guessing the right exposure time, while your time’s ...

Setting up a new Western blot?  Thanks to increased assay sensitivities, readily available antibody-coupling products and improved conjugate reagents, you have a big decision to make:  Should you do a direct or an indirect Western blot? The ...

Ever thought about using directly-labeled primary antibodies for your assay?  You can buy them commercially, or you can label them yourself.  With new kits and technology, it's fairly easy to do. Here are some reasons why you might want to add ...

Did you ever dream that as a scientist you would be a matchmaker? That's hardly what we were trained for. But if you want your ELISA to be happy, then you have to hone your skills in the art of l'amour. Yes, you will need to negotiate a ...