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Last week I took some time to reflect on how all the fine details of technique can impact the accuracy of my Western blots. These are all the little things that have become second nature to me over the years. After running thousands of Western ...

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There’s nothing more frustrating than ending your day with too many bands on your Western blot. Was it my sample? My blocker? My antibodies? If you are working with a new sample or antibody the answer is probably, I have no clue. Cue the painful ...

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Immunoprecipitation (IP) is a method used to enrich or purify your target protein from a complex mixture, such as a cell lysate, using a solid support. After IP you can analyze your samples by various techniques such as Western blot, ELISA or Mass ...

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