Posted on

Immunoprecipitation (IP) is a method used to enrich or purify your target protein from a complex mixture, such as a cell lysate, using a solid support. After IP you can analyze your samples by various techniques such as Western blot, ELISA or Mass ...

Continue reading »
Share:

Posted on

Loading control antibodies are a critical Western blotting tool that is often overlooked. They play an important role, ensuring that that you have loaded and transferred equivalent amounts of protein across your Western blot. If you are planning on ...

Continue reading »
Share:

Posted on

Selecting a primary antibody will be one of the most important decisions that you will make when designing your next experiment. Choose wisely because an antibody with poor specificity will wreak havoc on your data, making interpretation nearly ...

Continue reading »
Share:

Posted on

I love using fluorescent antibodies instead of the old-fashioned (well, I think they are) HRP-conjugated ones for Western Blots. If you work with dye-conjugated antibodies, you don't stress over guessing the right exposure time, while your time’s ...

Continue reading »
Share: