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Have membrane, will travel: Northwestern, Southwestern, Eastern blots
Ask Advansta: Should I use PVDF or Nitrocellulose Membrane?
A good sandwich must be tight – change your blotting pads
Direct fluorescent antibody labeling Q and A
Seeing Blue and Red: Coomassie vs. Ponceau staining

Posts Categorized: Ask Advansta

Ask Advansta: Should I use PVDF or Nitrocellulose Membrane?

Posted on June 13, 2016

Before you start your next Western, we can help you decide which membrane will be best for your experiment. The quick blot Start with a quick experiment that doesn't require the highest level of sensitivity. In this case, nitrocellulose is more ...

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Ask Advansta: Can I Use Ethanol to Activate a PVDF Membrane?

Posted on September 09, 2015

PVDF membranes are great for Western blots.  They bind protein readily, can take a beating from a strong-handed researcher and are ideal for fluorescent Western blots. But they do have an annoying quality that adds an extra step to your ...

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heat protein samples

Ask Advansta: Why Do We Heat Protein Samples Before Loading?

Posted on January 23, 2015

A few months ago, we launched one of our new products:  Visio, a protein loading buffer that allows you to watch your proteins as they migrate through the gel.  Compatible with downstream mass spectrometry, Visio contains a protein stain in the ...

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chemiluminescent western

Help! My Chemiluminescent Western Blot Didn’t Work!

Posted on August 13, 2014

Nothing is more frustrating in the lab than trying to troubleshoot a multi-step protocol. What do you do when you get to the end of your chemiluminescent Western and you have little to no signal? ...

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