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Buffers and solutions

Western Blot Strip-It Buffer
Western Blot Strip-It Buffer

Stripping buffer for stripping antibodies from chemiluminescent Western blots

  • Conserve valuable samples - probe the same blot multiple times
  • Fast protocol - blot stripped and ready for re-probing in 25 minutes
  • Gentle protocol - antibodies removed without harsh reagents or elevated temperatures
  • Save time - no need to re-run gels and perform duplicate blots
  • Versatile - compatible with both nitrocellulose and PVDF membranes and with a variety of chemiluminescent detection reagents
FLASHBlot transfer buffer
FLASHBlot transfer buffer

Ready-to-use transfer buffer
Designed for faster, more efficient protein transfer with increased sensitivity

FLASHBlot is not your average transfer buffer.

  • Faster than traditional buffers: better results in 20 minutes
  • More sensitive detection for low abundance proteins
  • Better detection of post-translationally modified proteins
  • Better transfer of ALL proteins, including high molecular weight proteins
  • Conveniently suitable for any wet transfer set-up

 

“ We were pleasantly surprised to see that with FLASHBlot we observed the same transferring levels at 25 min than with the lengthier transfer protocol… including for the 300 kDa protein. This reagent will shorten considerably our experimental time. ”  - Assistant Professor, UCSF

 

AdvanBlock-PF blocking solution
AdvanBlock-PF blocking solution

Non-protein blocking solution for fluorescent and chemiluminescent Western blotting

  • Low background - Protein-free block buffer for lower background with certain antibodies
  • Optimized - Stabilizes fluorescence of WesternBright MCF fluorescent secondary antibodies
  • Versatile - Compatible with fluorescent and chemiluminescent detection
AdvanBlock-Chemi blocking solution
AdvanBlock-Chemi blocking solution

Signal-enhancing Western blot block solution 
for the best chemiluminescent signals

  • Enhanced signal – greatly enhances Western blotting signals
  • Decreased background – decreased non-specific bands and lowered background
  • Sensitivity – get better signals for low protein sample concentrations
  • Convenience – Pre-made buffer means you save preparation and optimization time

AdvanBlock-EIA blocking solution
AdvanBlock-EIA blocking solution

Blocking solution for ELISAs and immunoassay plates

  • Decreases background
  • Decreases well-to-well variability
  • Decreases non-specific binding
  • Ready-to-use solution
AdvanBlock-Fluor blocking solution
AdvanBlock-Fluor blocking solution

Optimized block solution 
for the best fluorescent Western blot signals

  • Enhanced signal – enhances Western blotting signals
  • Decreased background – decreased non-specific bands and lowered background
  • Sensitivity – get better signals for low protein sample concentrations
  • Convenience – Pre-made buffer means you save preparation and optimization time
AdvanWash washing solution
AdvanWash washing solution

Washing solutions optimized for use with WesternBright systems

  • Reliable – quality controlled wash buffer for reproducible results
  • Versatile – compatible with chemiluminescent and fluorescent Western blots
  • Convenient – save time using pre-made buffers; no more weighing, mixing, or adjusting pH

 

10X EIA Coating Buffer
10X EIA Coating Buffer

Immunoassay plate coating solution

  • Reliable – quality-controlled coating buffer for reproducible results
  • Versatile – compatible with chemiluminescent and colorimetric EIA/ELISA assays
  • Convenient – save time using pre-made buffer. No more weighing, mixing or pH adjusting
Avant buffer pouches
Avant buffer pouches

Convenient, pure, fast buffer preparation

 

  • Quality – molecular biology-grade buffers of highest purity
  • Convenient – pre-measured powder in sealed pouches
  • Fast – dissolve and use right away
  • Standardized – guaranteed reproducible results for your electrophoresis gels and Western blots