I love using fluorescent antibodies instead of the old-fashioned (well, I think they are) HRP-conjugated ones for Western Blots. If you work with dye-conjugated antibodies, you don't stress over guessing the right exposure time, while your time’s ...

The Matrix: more than just a futuristic sci-fi movie, the matrix can have a confounding effect on your ELISA results.  Interactions between your protein of interest and other components in the sample, called matrix effects, can result in erroneous ...

If you want to run a quantitative ELISA assay, then you will need to include a standard curve on your plate. While this task might seem a bit mundane, an accurate standard curve can be hard to achieve. It requires careful handling of the standard, ...

Chemiluminescence isn't just for Western blots. You can make your ELISA shine too by using an ELISA substrate that produces light. In fact, we have the perfect substrate for sensitive, chemiluminescent-based detection for your ELISA assay: ...