In many labs, blocks for Western Blot, Dot Blot, and ELISA are “one size fits all” - one buffer and one blocking agent for any antibody. If the combination doesn’t work, people would rather give up than try changing the mix. So why do we ...

Summertime is over, but high outside temperature or not, you still need to defrost your samples for gels, Western blots and ELISAs. Let’s recap a good practice for freezing and thawing your samples. Each freeze/thaw cycle causes biomolecule ...

I love using fluorescent antibodies instead of the old-fashioned (well, I think they are) HRP-conjugated ones for Western Blots. If you work with dye-conjugated antibodies, you don't stress over guessing the right exposure time, while your time’s ...

The Matrix: more than just a futuristic sci-fi movie, the matrix can have a confounding effect on your ELISA results.  Interactions between your protein of interest and other components in the sample, called matrix effects, can result in erroneous ...