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Ordinarily we don’t like to recommend stripping and reprobing a Western blot.  A traditional stripping protocol results in uneven removal of proteins leading to loss of genuine signal, high background and unquantifiable data.

But we’ve changed our minds, and we think you will too.  Because Advansta now has a new solution for you (pun intended!).

Introducing Advansta’s Western Blot Strip-It Buffer – a proprietary solution that uniformly strips antibodies from Western blot membranes.

In an ideal world, you would always have enough sample to run as many blots as needed, your proteins of interest would separate well in the gel and your detection antibodies would always be compatible with one another.  But in the real world, you have to deal with precious samples, overlapping proteins and whatever antibodies you can find that recognize your proteins.

It leads many of us to want to strip and reprobe our blots, a process that is time-consuming and yes, we’ll admit it, stinky.

Now you can say good-bye to the stink, the hassle, the heat and the harsh treatment of your blots.

Advansta has developed Western Blot Strip-It Buffer, a mild stripping solution that quickly and uniformly removes antibodies from blots.

An example of the use of Strip-It Buffer is shown below.

Strip-It Buffer

Untreated HeLa cell lysate (4, 2, 1, and 0.5 μg) was separated by SDS-PAGE and transfered to nitrocellulose or PVDF membranes. Blots were probed with an anti-GAPDH antibody (A). After stripping with Western Blot Strip-It Buffer, no residual signal is detected (B). The blots were then re-probed with an anti-STAT1 antibody (C).

 Strip-It Buffer has the following advantages:

  • Allows you to conserve precious samples by probing the same blot multiple times
  • It saves time – strip blots in 25 minutes
  • A gentle protocol that removes antibodies without harsh reagents or high temperatures
  • Compatibility with both PVDF and nitrocellulose membranes as well as a variety of detection reagents
  • Comes in convenient 20 mL and 500 mL sizes

With Strip-It, you don’t have to dread reprobing your blot.

Featured image courtesy of Mary-Frances Main.

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