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New to fluorescence imaging systems?  Or maybe you are considering buying a new one?  Don’t you wish there was an easy way to evaluate the performance of a fluorescent imager (other than wasting your time running your own Western blot)?

Well there is!

Thanks to the durability of fluorescent signals, you can buy a pre-processed, standardized blot to evaluate three different fluorescent channels on your imager: red, green and blue.

The Advansta Fluorescent Western Standardization blot can be used to demonstrate the performance of laser scanners and CCD-based systems.

Each blot contains three proteins (alpha-fetoprotein, AFP; carcinoembryonic antigen, CEA; and transferrin) at different ratios in each lane. The blots are probed with a mixture of primary antibodies raised in mouse and rabbit, and stained with a mixture of secondary antibodies labeled with different fluorescent dyes. AFP is detected in the green channel, CEA is detected in the red channel and transferrin is detected by a goat primary antibody directly labeled with a dye that can be detected in the blue channel. The amount of transferrin is constant in all lanes. while AFP and CEA vary according to the chart at the bottom.

The advantages of the standardized blot include:

  • Convenience
    • The blot is the easiest way to evaluate the performance of your fluorescence imaging system
  • Reliability
    • Each blot is quality-controlled with lot-specific statistics provided for every blot
  • Flexible design
    • Both fluorescently-labeled primary and secondary antibodies are used to demonstrate different experimental designs
chart 5

Protein amount in ng; MW – molecular weight markers

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