PVDF membranes are great for Western blots.  They bind protein readily, can take a beating from a strong-handed researcher and are ideal for fluorescent Western blots. But they do have an annoying quality that adds an extra step to your ...

Larger proteins can be notoriously difficult to work with when Western blotting.  In particular, you can have a hard time achieving a good transfer efficiency.  In some ways,   large proteins are like teenagers:  they are gangly and awkward, ...

For most proteins, standard SDS-PAGE works well to separate the molecules prior to downstream applications, such as Western blotting.  But what if you are working with small molecular weight proteins, like something smaller than 10kDa (cringe)?  ...

Most people know that gel electrophoresis separates proteins based on charge and size. But the type of gel you run really determines how your proteins are separated and can affect your outcome. Denaturing and native gels are not ...