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Have membrane, will travel: Northwestern, Southwestern, Eastern blots
Ask Advansta: Should I use PVDF or Nitrocellulose Membrane?
A good sandwich must be tight – change your blotting pads
Direct fluorescent antibody labeling Q and A
Seeing Blue and Red: Coomassie vs. Ponceau staining

Posts Categorized: Western blots

heat protein samples

Ask Advansta: Why Do We Heat Protein Samples Before Loading?

Posted on January 23, 2015

A few months ago, we launched one of our new products:  Visio, a protein loading buffer that allows you to watch your proteins as they migrate through the gel.  Compatible with downstream mass spectrometry, Visio contains a protein stain in the ...

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phosphorylated proteins

8 Tips for Detecting Phosphorylated Proteins by Western Blot

Posted on January 20, 2015

Detecting phosphorylated proteins on a Western blot isn't really different from doing a regular Western blot.  But there are a few tricks that can help you get the perfect blot: ...

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protein resolution

Need Better Protein Resolution? Change Your Buffers!

Posted on January 16, 2015

When most of us make our first foray into running proteins on a gel, we use a standard Tris-glycine gel.  And why not?  Unless you are running a very large protein or a little itty-bitty protein, you probably get pretty good protein ...

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western or elisa

Eeny, Meeny, Miny, Moe – Western or ELISA, Which Way Should I Go?

Posted on January 14, 2015

Western or ELISA  – when you think about it, they are really they same assay. You stick some protein down to a support, incubate with antibodies and use a substrate that produces color, light or fluorescence to detect a protein of ...

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