Have you ever had to dialyze protein? Your protein is in a buffer not compatible with SDS-PAGE and you need to run it on a gel. So you resort to dialysis. You squirt your protein solution into a membrane tube, hope the clamps on either end hold, ...

In my various laboratory experiences (N=5; probably not enough to achieve any kind of statistical significance) it seems that labs either use a Bradford or a BCA protein assay to quantify protein concentrations. Both assays come in convenient ...

Us scientists live up to our Frankenstein-producing alter egos when it comes to breaking down cells. We have almost as many ways to lyse cells as grant reviewers have of saying no. The question we ask today is: How do you grind your cells? ...

Detergent lysis couldn't be any easier, right? You rinse your cells, throw on some lysis buffer, scrape or pipette the cells into solution and you are ready to go! But which detergent do you put in your lysis buffer? Think carefully because the ...