When most of us make our first foray into running proteins on a gel, we use a standard Tris-glycine gel.  And why not?  Unless you are running a very large protein or a little itty-bitty protein, you probably get pretty good protein ...

Some studies suggest that up to 30% of proteins are phosphorylated in eukaryotic cells.  Whoa. So chances are, if you study proteins, you are going to be examining protein phosphorylation at some point in your career.  But how do you know which ...

So you've decided to take the plunge and go for a total stain to normalize your Western blot. With some beginner's luck, it might turn out better than you have ever imagined.....or it might need some improvement. Try these helpful tips to improve ...

Normalizing your Western blot is critical to obtaining accurate quantitative measurements. But it can be tricky. Perhaps you are discovering that housekeeping genes are not as neat and tidy as you would like? Then you might like to try total protein ...