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Us scientists live up to our Frankenstein-producing alter egos when it comes to breaking down cells. We have almost as many ways to lyse cells as grant reviewers have of saying no. The question we ask today is: How do you grind your cells? ...

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Detergent lysis couldn't be any easier, right? You rinse your cells, throw on some lysis buffer, scrape or pipette the cells into solution and you are ready to go! But which detergent do you put in your lysis buffer? Think carefully because the ...

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Photo courtesy of erin. What is one of the most simplest things you can do for protein isolation to ensure a good prep? Keep it cool, man. Proteases, those sneaky, protein-nibbling enzymes, are more active at higher temperatures. Keeping ...

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