Detergent lysis couldn't be any easier, right? You rinse your cells, throw on some lysis buffer, scrape or pipette the cells into solution and you are ready to go! But which detergent do you put in your lysis buffer? Think carefully because the ...

Photo courtesy of Steampunk Family the von Hedwigs Cocktails in the lab? Normally I wouldn't recommend it. But sometimes a good cocktail is what you need to get the job done right. When you lyse cells – either by adding detergents or by ...

Photo courtesy of smaller.pathological.ca If you work with yeast, then you will get a totally different meaning out of that title :) However, if you spend a lot of time doing antibody-based assays then you will know what I am talking ...

Photo courtesy of Raymond Bryson Troubleshooting and optimizing Western blots can eat up a lot of your time. Transfer conditions, blocking buffers, primary and secondary antibody titrations - there are so many things that can be ...