In the spirit of the Halloween season, it's time to let our skeletons out of the lab closet and share some of those freaky Western blots. You know – those scary ones that you tuck away in the back of your notebook hoping you never have to look at ...

It's tradition – you perform a Western blot to observe the expression pattern of your protein of interest. Then, you also probe for housekeeping genes to prove that any changes you see are not due to loading inconsistencies nor a lack of protein ...

Stripping – the quick and dirty way to maximize the results from a single Western blot. It is tempting to use this technique to probe for an additional protein. But don't be seduced by this seemingly innocuous step. While there may be a time when ...

Western blot film – some is blue some is gray.  Some is even clear.  Then there are the boxes of pre-exposed film with black edges and corners that you only use when you have no other film left. Does it make a difference which film you use ...