When it comes to quantitative fluorescent Western blotting, more is definitely not always better. Reliable quantitative Western blot data can only be attained by loading the proper amount of sample. Loading too much protein can result in signal ...

Since the Western blot was invented back in 1979, little has changed to make the process better, easier or faster. When it comes to Western blotting, nothing is more soul crushing than spending 1–2 days running a Western, only to find out that ...

Back in the 1970’s, Edwin Southern came up with an ingenious method to study DNA fragments. By integrating three key innovations - restriction enzymes, gel electrophoresis and blotting techniques he greatly increased the speed and simplicity of ...

Before you start your next Western, we can help you decide which membrane will be best for your experiment. The quick blot Start with a quick experiment that doesn't require the highest level of sensitivity. In this case, nitrocellulose is more ...