Western blotting technical resources
Western blotting is a multi-step procedure which can require optimization of numerous factors. Advansta’s guide to Protein Analysis includes advice for optimizing numerous variables including:
- Polyacrylamide percentage of gel for best protein separation
- Amount of protein loaded onto gel
- Selection of appropriate transfer membrane
- Dilution of primary and secondary antibodies
- Blocking conditions
- Detection methods
Download the Protein Analysis guide here:
Download Advansta’s Step-by-Step Guide to Western Blots here:
Advansta’s Step-by-Step Guide to Western Blots
Download the Advansta’s Step-by-Step Guide to In-Cell Westerns here:
Advansta’s Step-by-Step Guide to In-Cell Westerns
Download the Electrophoresis and Western Blotting Layout here (available in full page or half page sizes):
Electrophoresis and Western Blotting Layout (full page)
Electrophoresis and Western Blotting Layout (half page)
Advansta’s Western blot detection reagents
For advice related to specific Advansta Western blotting detection systems, please refer to the user manuals for each product:
WesternBright Quantum user manual
WesternBright Sirius user manual
Or refer to the Advansta FAQ page to find answers to frequently asked questions.
General Western blotting troubleshooting
For a guide to trouble shooting the entire process, from sample preparation to electrophoresis to imaging, see our Western blotting troubleshooting page.
