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FLASHBlot transfer buffer

Ready-to-use wet transfer buffer
Designed for faster, more efficient wet protein transfer with increased sensitivity

FLASHBlot is not your average wet transfer buffer.

  • Faster than traditional wet buffers: better results in 20 minutes
  • More sensitive detection for low abundance proteins
  • Better detection of post-translationally modified proteins
  • Better transfer of ALL proteins, including high molecular weight proteins
  • Conveniently suitable for any wet transfer set-up

“ We were pleasantly surprised to see that with FLASHBlot we observed the same transferring levels at 25 min than with the lengthier transfer protocol… including for the 300 kDa protein. This reagent will shorten considerably our experimental time. ”  - Assistant Professor, UCSF

 

FLASHBlot transfer buffer


CAT # PRODUCT SIZE PRICE QUANTITY
R-03090-D25 FlashBlot Transfer Buffer (50x) 250 ml $ 95.00
(USD)
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R-03090-D50 FlashBlot Transfer Buffer (50x) 500 ml $ 159.00
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FlashBlot-SD™ Semi-Dry Transfer Buffer (1x) 500 ml $ 112.00
(USD)
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Description

Compared to traditional wet transfer buffers, FLASHBlot Transfer Buffer provides improved transfer efficiency of all proteins, including those of high molecular weight for which is it often very difficult to achieve complete transfer.

FLASHBlot Figure 1
Complete transfer in less than 20 minutes. Transfer of CEA (high MW) and GAPDH (low MW) proteins was compared using FLASHBlot and Towbin transfer buffers. Following Western blot analysis, the data show equivalent protein transfer in 15 minutes with FLASHBlot vs. 60 minutes with Towbin buffer.

Due to the faster transfer time, FLASHBlot Transfer Buffer allows for increased protein retention on membranes resulting in more sensitive detection of low-abundance proteins.

FLASHBlot Figure 2
Shorter transfer times. FLASHBlot achieves complete protein transfer in less than 20 minutes using any traditional wet transfer apparatus.
FLASHBlot Figure 3
More efficient transfers with FLASHBlot buffer. After 20-min transfers, Western blot analysis shows that both CEA and GAPDH are more efficiently tranferred using FLASHBlot buffer than using Towbin buffer.

The short transfer times - plus increased transfer efficiency and protein retention - allow for sensitive detection of posttranslationally modified proteins, which can be difficult to detect after traditional transfer protocols.

FLASHBlot Figure 2
Superb detection of post-translationally modified proteins of interest. Western blot analysis of untreated and IFNα treated HeLa cell lysate using STAT1 and phospho-STAT1 shows efficiency of FlashBLOT Transfer Buffer. The blot was imaged with a CCD camera.

 

FLASHBlot-SD transfer buffer

Rapid high efficiency semi-dry transfer buffer

FLASHBlot-SD Transfer Buffer is designed for rapid semi-dry transfer of proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using rapid semi-dry transfer systems. Transfer is compatible with commonly used detection methods such as membrane staining, chemiluminescent and fluorescent Western blotting.

  • Fast - high ionic strength formulation allows for protein transfer in 3 to 10 minutes when used with a compatible high current semi-dry system
  • Compatible - use your existing high current semi-dry transfer apparatus
  • Reproducible - consistent transfer across entire blot
  • Versatile - use nitrocellulose or PVDF membranes to achieve transfers with low background and high sensitivity with both chemiluminescent and fluorescent Western blots
FLASHBlot-SD Figure 2
FLASHBlot-SD produces fluorescent Western blots with low background and high sensitivity. IR fluorescent Western blot analysis of phosphorylated STAT3 and GAPDH was performed on blots containing serially diluted HeLa lysate (±IFNα treatment) that was electrophoresed by SDS-PAGE then transferred to PVDF membranes. Proteins were transferred from gel to membrane for 7 minutes at a constant current of 1.3 Amps.
FLASHBlot-SD Figure 3
FLASHBlot-SD produces chemiluminescent Western blots with highest sensitivity. Chemiluminescent Western blot analysis of hnRNP K was performed on blots containing serially diluted HeLa lysate that was electrophoresed by SDS-Page then transferred to nitrocellulose membranes. Proteins were transferred from gel to membrane for 7 minutes at a constant current of 1.3 Amps.
FLASHBlot-SD Figure 5
FLASHBlot-SD outperforms competitive semi-dry transfer buffers. (a) AdvanStain Total-PVDF fluorescent protein membrane staining was performed on blots containing serially diluted HeLa lysate that was electrophoresed by SDS-PAGE then transferred to PVDF membranes. Proteins were transferred from gel to membrane for 7 minutes at a constant current of 1.3 Amps. (b) After membrane staining was complete, an IR800 fluorescent Western blot analysis of GAPDH was performed. FLASHBlot-SD produced 4-8 fold higher Western blot sensitivity in comparison to other commercially available transfer buffers.