Chemiluminescent Western blotting

Convenient nitrocellulose and PVDF membranes for Western blotting

• Performance – quality controlled for background-free Western blots
• Convenience – membranes available pre-cut and as rolls
• High signal-to-noise ratio – low background signal for clearer results
• Flexibility – choose the right membrane for your Western blotting application

Membrane Options:

WesternBright PVDF-FL: fluorescent detection
WesternBright PVDF-CL: chemiluminescent detection; best stability for stripping and re-probing chemiluminescent blots
PVDF membranes available as rolls or pre-cut (7x9 cm, 10x15 cm, or 13x18 cm)

WesternBright NC: all-purpose nitrocellulose membrane
Nitrocellulose membranes available as rolls or pre-cut (8x10 cm)

A chemiluminescent marker for writing on membranes

• Improve accuracy - transform your visible protein ladder standard into a chemiluminescent standard on your Western blot
• Annotate - permanently mark your blots with the date or blot ID
• Compatible - with both X-ray film and CCD imagers
• Confirm HRP substrate stability - the ChemiPen reagent glows when incubated with an HRP substrate, so it can be used to test substrate stability

The best choice for routine, film-imaged Western blots

• Easy to use - just point and spray. No measuring or mixing required
• Stronger signal than Amersham™ ECL or Pierce™ ECL
• Sensitive - detect low pg protein amounts
• Save - use up to ten-times less antibody

Includes:
WesternBright ECL Luminol/enhancer solution
WesternBright Peroxide Chemiluminescent peroxide solution

The most sensitive chemiluminescent substrate

• Most sensitive of Advansta's HRP substrates, for low-abundance proteins
• Long-lasting signal for flexible imaging
• Low background for high signal-to-noise
• Image by CCD or film

Includes:
WesternBright™ Sirius™Luminol/enhancer solution
WesternBright™ Peroxide Chemiluminescent Detection Reagent