Chemiluminescent Western blotting

FLASHBlot-SD transfer buffer
FLASHBlot-SD transfer buffer

Rapid high efficiency semi-dry transfer buffer

  • Fast - high ionic strength formulation allows for protein transfer in 3 to 10 minutes when used with a compatible high current semi-dry system
  • Compatible - use your existing high current semi-dry transfer apparatus
  • Reproducible - consistent transfer across entire blot
  • Versatile - use nitrocellulose or PVDF membranes to achieve transfers with low background and high sensitivity with both chemiluminescent and fluorescent Western blots
Western Blot Strip-It Buffer
Western Blot Strip-It Buffer

Stripping buffer for stripping antibodies from chemiluminescent Western blots

  • Conserve valuable samples - probe the same blot multiple times
  • Fast protocol - blot stripped and ready for re-probing in 25 minutes
  • Gentle protocol - antibodies removed without harsh reagents or elevated temperatures
  • Save time - no need to re-run gels and perform duplicate blots
  • Versatile - compatible with both nitrocellulose and PVDF membranes and with a variety of chemiluminescent detection reagents

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LucentBlue X-ray film
LucentBlue X-ray film

High sensitivity X-ray film
for chemiluminescent and other blots

  • Performance - excellent images when used with WesternBright ECL
  • Value - sensitivity equal to more expensive films
  • Flexibility - detect radioisotopes in addition to chemiluminescence
AdvanStain Iris
AdvanStain Iris

High sensitivity stain for Nitrocellulose and PVDF Membranes

 

  • Sensitive – detect as little as 2–10ng per band
  • Rapid – detect proteins in 20 minutes or less
  • Compatible – Western blot ready, no destaining required

AdvanBlock-Chemi blocking solution
AdvanBlock-Chemi blocking solution

Signal-enhancing Western blot block solution 
for the best chemiluminescent signals

  • Enhanced signal – greatly enhances Western blotting signals
  • Decreased background – decreased non-specific bands and lowered background
  • Sensitivity – get better signals for low protein sample concentrations
  • Convenience – Pre-made buffer means you save preparation and optimization time

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AdvanWash washing solution
AdvanWash washing solution

Washing solutions optimized for use with WesternBright systems

  • Reliable – quality controlled wash buffer for reproducible results
  • Versatile – compatible with chemiluminescent and fluorescent Western blots
  • Convenient – save time using pre-made buffers; no more weighing, mixing, or adjusting pH

 

AdvanStain Total Fluorescent Protein Staining Kits
AdvanStain Total Fluorescent Protein Staining Kits

Rapid fluorescent stain for total protein normalization

 

  • Convenient – simple protocol that takes less than 20 minutes, no de-staining required
  • Versatile – compatible with chemiluminescent and IR700/IR800 fluorescent Western blotting
  • Accurate – linear across a broad range from 1-50μg of lysate, ideal for total protein normalization

WesternEaze-Chemi Kit
WesternEaze-Chemi Kit

Rapid, high sensitivity chemiluminescent Western blot kit for protein detection

 

  • Rapid – complete Western blot in 1 hour
  • Reproducible – fewer hands-on manipulations equate to consistent data
  • Convenient – contains all critical reagents required to perform a chemiluminescent Western
  • Versatile – kits are available for both mouse and rabbit primary antibodies

X-ray film cassette
X-ray film cassette

Light-tight film cassette for exposing blots

  •  Convenient - quickly load and unload with simple push-button latch
  • Light weight - quality light-weight aluminum construction
  • Proven performance - no light leaks even with overnight exposures
WesternBright ECL Spray
WesternBright ECL Spray

The best choice for routine, film-imaged Western blots

  • Easy to use - just point and spray. No measuring or mixing required
  • Stronger signal than Amersham™ ECL or Pierce™ ECL
  • Sensitive - detect low pg protein amounts
  • Save - use up to ten-times less antibody

 

Includes:
WesternBright ECL Luminol/enhancer solution
WesternBright Peroxide Chemiluminescent peroxide solution

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