The best results for Western blots are obtained when both the primary and secondary antibodies are accurately titrated. Although dot blots cannot determine the molecular weight or integrity of a protein and therefore should never be used to identify ...

Sample preparation, loading, uneven transfer and transfer efficiency can all affect recovery and detection of proteins on the membrane. When evaluating the amount of protein of interest on the blot, it is vital to use a normalization control. ...

After lysis of cells, it is important to determine the total protein concentration of the sample. Accurate quantitation of the sample will allow you to load the proper amount of protein in each lane. This avoids overloading the lane but still allows ...

Efficient cell lysis is an important step in preparing quality samples for Western blot analysis. Inefficient lysis can result in incomplete isolation and recovery of proteins, decreasing the ability to detect the protein of interest. Sample ...