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Light-tight film cassette for exposing blots
- Convenient - quickly load and unload with simple push-button latch
- Light weight - quality light-weight aluminum construction
- Proven performance - no light leaks even with overnight exposures
Rapid, high sensitivity chemiluminescent Western blot kit for protein detection
- Rapid – complete Western blot in 1 hour
- Reproducible – fewer hands-on manipulations equate to consistent data
- Convenient – contains all critical reagents required to perform a chemiluminescent Western
- Versatile – kits are available for both mouse and rabbit primary antibodies
The most sensitive chemiluminescent substrate
- Most sensitive of Advansta's HRP substrates, for low-abundance proteins
- Long-lasting signal for flexible imaging
- Low background for high signal-to-noise
- Image by CCD or film
Includes:
WesternBright™ Sirius™Luminol/enhancer solution
WesternBright™ Peroxide Chemiluminescent Detection Reagent
Quantitative chemiluminescent Western blotting
- Sensitive – detect attomoles of protein per band.
- Quantitative – linear range of signal with respect to protein amount exceeds 3 orders of magnitude
- Versatile – Optimized for CCD imaging, and compatible with film detection. Chemifluorescent emissions can be detected with fluorescence imaging systems
- Low background – for high signal to noise ratio
- Long lasting signal – image blots hours after substrate incubation
Includes:
WesternBright Quantum luminol/enhancer solution
WesternBright Quantum stabilized peroxide solution
The best choice for routine, film-imaged Western blots
- Easy to use - just point and spray. No measuring or mixing required
- Stronger signal than Amersham™ ECL or Pierce™ ECL
- Sensitive - detect low pg protein amounts
- Save - use up to ten-times less antibody
Includes:
WesternBright ECL Luminol/enhancer solution
WesternBright Peroxide Chemiluminescent peroxide solution
The best choice for routine, film-imaged chemiluminescent Western blots
- Stronger signal than Amersham™ ECL or Pierce™ ECL
- Optimized – for x-ray film imaging
- Sensitive – detect low pg protein amounts
- Lasting signal – up to 6 hrs
- Save money – use up to 10x less antibody
Includes:
WesternBright ECL Luminol/enhancer solution
WesternBright Peroxide Chemiluminescent peroxide solution
A chemiluminescent marker for writing on membranes
- Improve accuracy - transform your visible protein ladder standard into a chemiluminescent standard on your Western blot
- Annotate - permanently mark your blots with the date or blot ID
- Compatible - with both X-ray film and CCD imagers
- Confirm HRP substrate stability - the ChemiPen reagent glows when incubated with an HRP substrate, so it can be used to test substrate stability
Stripping buffer for stripping antibodies from chemiluminescent Western blots
- Conserve valuable samples - probe the same blot multiple times
- Fast protocol - blot stripped and ready for re-probing in 25 minutes
- Gentle protocol - antibodies removed without harsh reagents or elevated temperatures
- Save time - no need to re-run gels and perform duplicate blots
- Versatile - compatible with both nitrocellulose and PVDF membranes and with a variety of chemiluminescent detection reagents
Convenient nitrocellulose and PVDF membranes for Western blotting
- Performance – quality controlled for background-free Western blots
- Convenience – membranes available pre-cut and as rolls
- High signal-to-noise ratio – low background signal for clearer results
- Flexibility – choose the right membrane for your Western blotting application
Membrane Options:
WesternBright PVDF-FL: fluorescent detection
WesternBright PVDF-CL: chemiluminescent detection; best stability for stripping and re-probing chemiluminescent blots
PVDF membranes available as rolls or pre-cut (7x9 cm, 10x15 cm, or 13x18 cm)
WesternBright NC: all-purpose nitrocellulose membrane
Nitrocellulose membranes available as rolls or pre-cut (8x10 cm)
High sensitivity X-ray film
for chemiluminescent and other blots
- Performance - excellent images when used with WesternBright ECL
- Value - sensitivity equal to more expensive films
- Flexibility - detect radioisotopes in addition to chemiluminescence
Convenient containers for staining and washing gels and membranes
- Smooth interior – protects membranes and gels from scratches
- Convenient – multiple sizes (5x7, 6x9, 9x11,10.5x15.5 cm) and designs available for different gels and blots
- Attached lid – to protect experiments from dust or debris that can cause speckles on blot images
- Save – minimize antibody and buffer usage by using appropriately sized containers
- Multi-chamber trays – process 6 mini-blots simultaneously, each chamber requires as little as 3 mL of solution
HRP-conjugated secondary antibodies
for chemiluminescent immunodetection
- Performance – excellent signal in immunoblotting and ELISA applications
- Convenience – each antibody is demonstrated to provide optimal results with Advansta's protein detection systems
- Purity – each antibody is affinity purified to maximize specificity
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