Chemiluminescent Western blotting

WesternBright ECL HRP substrate
WesternBright ECL HRP substrate

The best choice for routine, film-imaged chemiluminescent Western blots

  • Stronger signal than Amersham™ ECL or Pierce™ ECL
  • Optimized – for x-ray film imaging
  • Sensitive – detect low pg protein amounts
  • Lasting signal – up to 6 hrs
  • Save money – use up to 10x less antibody

 

Includes:
WesternBright ECL Luminol/enhancer solution
WesternBright Peroxide Chemiluminescent peroxide solution

 

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Blotting sponge pads
Blotting sponge pads

Transfer sponge pads for more reproducible Western blot transfers

  • Resist compression - maintain proper pressure in transfer sandwiches without adding extra blotting papers
  • Retain thickness - can be reused over multiple experiments
  • Convenient - two sizes (8 x 11 cm and 9 x 15 cm) compatible with a variety of transfer apparatuses
  • Standardized - uniform flat construction ensures reproducible and consistent transfers
Incubation trays
Incubation trays

Convenient containers for staining and washing gels and membranes

  • Smooth interior – protects membranes and gels from scratches
  • Convenient – multiple sizes (5x7, 6x9, 9x11,10.5x15.5 cm) and designs available for different gels and blots
  • Attached lid – to protect experiments from dust or debris that can cause speckles on blot images
  • Save – minimize antibody and buffer usage by using appropriately sized containers
  • Multi-chamber trays – process 6 mini-blots simultaneously, each chamber requires as little as 3 mL of solution

 

Blotting papers
Blotting papers

Pre-cut paper for electrophoretic transfers

  • Convenient – pre-cut; work with most mini gel transfer assemblies
  • Standardized – uniform thickness for reproducible transfer
  • Tested – perform consistently without artifacts or background
  • Available sizes include 7x9 cm and 8x10 cm
Development folders
Development folders

Transparent plastic supports for handling and imaging blots

  • Clear images – avoid the wrinkling or bunching that can happen with plastic wrap
  • Suitable for all blots – development folders are suitable for chemiluminescence and fluorescence imaging
  • Safe blot handling – move your blot without scratching or putting pressure on the surface
  • Convenient size – image up to 2 blots simultaneously in one folder
Background Quenching Sheets
Background Quenching Sheets

An added layer to decrease background
For improved imaging of blots and gels

  • Better images by removing background fluorescence and stray light
  • Improved signal to noise ratios
  • Simple – just place sheet under the blot or gel
  • Compatible with chemiluminescent and fluorescent Western blots
  • Compatible with DNA gels and protein stains - including SYBR green, SYBR Gold, ethidium bromide, SYPRO® Orange, SYPRO Ruby and more

 

WesternBright Quantum HRP substrate
WesternBright Quantum HRP substrate

Quantitative chemiluminescent Western blotting

  • Sensitive – detect attomoles of protein per band.
  • Quantitative – linear range of signal with respect to protein amount exceeds 3 orders of magnitude
  • Versatile – Optimized for CCD imaging, and compatible with film detection. Chemifluorescent emissions can be detected with fluorescence imaging systems
  • Low background – for high signal to noise ratio
  • Long lasting signal – image blots hours after substrate incubation

 

Includes:
WesternBright Quantum luminol/enhancer solution
WesternBright Quantum stabilized peroxide solution

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WesternBright Sirius HRP substrate
WesternBright Sirius HRP substrate

The most sensitive chemiluminescent substrate

  • Most sensitive of Advansta's HRP substrates, for low-abundance proteins
  • Long-lasting signal for flexible imaging
  • Low background for high signal-to-noise
  • Image by CCD or film

Includes:
WesternBright™ Sirius™Luminol/enhancer solution
WesternBright™ Peroxide Chemiluminescent Detection Reagent

 

 

 

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Transfer membranes
Transfer membranes

Convenient nitrocellulose and PVDF membranes for Western blotting

  • Performance – quality controlled for background-free Western blots
  • Convenience – membranes available pre-cut and as rolls
  • High signal-to-noise ratio – low background signal for clearer results
  • Flexibility – choose the right membrane for your Western blotting application

 

Membrane Options:

WesternBright PVDF-FL: fluorescent detection
WesternBright PVDF-CL: chemiluminescent detection; best stability for stripping and re-probing chemiluminescent blots
PVDF membranes available as rolls or pre-cut (7x9 cm, 10x15 cm, or 13x18 cm)

WesternBright NC: all-purpose nitrocellulose membrane
Nitrocellulose membranes available as rolls or pre-cut (8x10 cm)

WesternBright ChemiPen
WesternBright ChemiPen

A chemiluminescent marker for writing on membranes

  • Improve accuracy - transform your visible protein ladder standard into a chemiluminescent standard on your Western blot
  • Annotate - permanently mark your blots with the date or blot ID
  • Compatible - with both X-ray film and CCD imagers
  • Confirm HRP substrate stability - the ChemiPen reagent glows when incubated with an HRP substrate, so it can be used to test substrate stability
FLASHBlot transfer buffer
FLASHBlot transfer buffer

Ready-to-use transfer buffer
Designed for faster, more efficient protein transfer with increased sensitivity

FLASHBlot is not your average transfer buffer.

  • Faster than traditional buffers: better results in 20 minutes
  • More sensitive detection for low abundance proteins
  • Better detection of post-translationally modified proteins
  • Better transfer of ALL proteins, including high molecular weight proteins
  • Conveniently suitable for any wet transfer set-up

 

“ We were pleasantly surprised to see that with FLASHBlot we observed the same transferring levels at 25 min than with the lengthier transfer protocol… including for the 300 kDa protein. This reagent will shorten considerably our experimental time. ”  - Assistant Professor, UCSF

 

HRP-conjugated secondary antibodies
HRP-conjugated secondary antibodies

HRP-conjugated secondary antibodies
for chemiluminescent immunodetection

  • Performance – excellent signal in immunoblotting and ELISA applications
  • Convenience – each antibody is demonstrated to provide optimal results with Advansta's protein detection systems
  • Purity – each antibody is affinity purified to maximize specificity