Solutions for protein characterization : Chemiluminescent Western...

Chemiluminescent Western blotting

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Western Blot Strip-It Buffer

Stripping buffer for stripping antibodies from chemiluminescent Western blots

Conserve valuable samples - probe the same blot multiple times
Fast protocol - blot stripped and ready for re-probing in 25 minutes
Gentle protocol - antibodies removed without harsh reagents or elevated temperatures
Save time - no need to re-run gels and perform duplicate blots
Versatile - compatible with both nitrocellulose and PVDF membranes and with a variety of chemiluminescent detection reagents

Transfer membranes

Convenient nitrocellulose and PVDF membranes for Western blotting

Performance – quality controlled for background-free Western blots
Convenience – membranes available pre-cut and as rolls
High signal-to-noise ratio – low background signal for clearer results
Flexibility – choose the right membrane for your Western blotting application

Membrane Options:

WesternBright PVDF-FL: fluorescent detection
WesternBright PVDF-CL: chemiluminescent detection; best stability for stripping and re-probing chemiluminescent blots
PVDF membranes available as rolls or pre-cut (7x9 cm, 10x15 cm, or 13x18 cm)

WesternBright NC: all-purpose nitrocellulose membrane
Nitrocellulose membranes available as rolls or pre-cut (8x10 cm)

Development folders

Transparent plastic supports for handling and imaging blots

Clear images – avoid the wrinkling or bunching that can happen with plastic wrap
Suitable for all blots – development folders are suitable for chemiluminescence and fluorescence imaging
Safe blot handling – move your blot without scratching or putting pressure on the surface
Convenient size – image up to 2 blots simultaneously in one folder

LucentBlue X-ray film

High sensitivity X-ray film
for chemiluminescent and other blots

Performance - excellent images when used with WesternBright ECL
Value - sensitivity equal to more expensive films
Flexibility - detect radioisotopes in addition to chemiluminescence

Blotting sponge pads

Transfer sponge pads for more reproducible Western blot transfers

Resist compression - maintain proper pressure in transfer sandwiches without adding extra blotting papers
Retain thickness - can be reused over multiple experiments
Convenient - two sizes (8 x 11 cm and 9 x 15 cm) compatible with a variety of transfer apparatuses
Standardized - uniform flat construction ensures reproducible and consistent transfers
Four pads per pack - a full set for common transfer apparatus

AdvanBlock-Chemi blocking solution

Signal-enhancing Western blot block solution
for the best chemiluminescent signals

Enhanced signal – greatly enhances Western blotting signals
Decreased background – decreased non-specific bands and lowered background
Sensitivity – get better signals for low protein sample concentrations
Convenience – Pre-made buffer means you save preparation and optimization time

WesternBright Quantum HRP substrate

Quantitative chemiluminescent Western blotting

Sensitive – detect attomoles of protein per band.
Quantitative – linear range of signal with respect to protein amount exceeds 3 orders of magnitude
Versatile – Optimized for CCD imaging, and compatible with film detection. Chemifluorescent emissions can be detected with fluorescence imaging systems
Low background – for high signal to noise ratio
Long lasting signal – image blots hours after substrate incubation

Includes:
WesternBright Quantum luminol/enhancer solution
WesternBright Quantum stabilized peroxide solution

Incubation trays

Convenient containers for staining and washing gels and membranes

Smooth interior – protects membranes and gels from scratches
Convenient – multiple sizes (5x7, 6x9, 9x11,10.5x15.5 cm) and designs available for different gels and blots
Attached lid – to protect experiments from dust or debris that can cause speckles on blot images
Save – minimize antibody and buffer usage by using appropriately sized containers
Multi-chamber trays – process 6 mini-blots simultaneously, each chamber requires as little as 3 mL of solution