Chemiluminescent Western blotting

LucentBlue X-ray film
LucentBlue X-ray film

High sensitivity X-ray film
for chemiluminescent and other blots

  • Performance - excellent images when used with WesternBright ECL
  • Value - sensitivity equal to more expensive films
  • Flexibility - detect radioisotopes in addition to chemiluminescence
Background Quenching Sheets
Background Quenching Sheets

An added layer to decrease background
For improved imaging of blots and gels

  • Better images by removing background fluorescence and stray light
  • Improved signal to noise ratios
  • Simple – just place sheet under the blot or gel
  • Compatible with chemiluminescent and fluorescent Western blots
  • Compatible with DNA gels and protein stains - including SYBR green, SYBR Gold, ethidium bromide, SYPRO® Orange, SYPRO Ruby and more

 

WesternBright ChemiPen
WesternBright ChemiPen

A chemiluminescent marker for writing on membranes

  • Improve accuracy - transform your visible protein ladder standard into a chemiluminescent standard on your Western blot
  • Annotate - permanently mark your blots with the date or blot ID
  • Compatible - with both X-ray film and CCD imagers
  • Confirm HRP substrate stability - the ChemiPen reagent glows when incubated with an HRP substrate, so it can be used to test substrate stability
Transfer membranes
Transfer membranes

Convenient nitrocellulose and PVDF membranes for Western blotting

  • Performance – quality controlled for background-free Western blots
  • Convenience – membranes available pre-cut and as rolls
  • High signal-to-noise ratio – low background signal for clearer results
  • Flexibility – choose the right membrane for your Western blotting application

 

Membrane Options:

WesternBright PVDF-FL: fluorescent detection
WesternBright PVDF-CL: chemiluminescent detection; best stability for stripping and re-probing chemiluminescent blots
PVDF membranes available as rolls or pre-cut (7x9 cm, 10x15 cm, or 13x18 cm)

WesternBright NC: all-purpose nitrocellulose membrane
Nitrocellulose membranes available as rolls or pre-cut (8x10 cm)

Incubation trays
Incubation trays

Convenient containers for staining and washing gels and membranes

  • Smooth interior – protects membranes and gels from scratches
  • Convenient – multiple sizes (5x7, 6x9, 9x11,10.5x15.5 cm) and designs available for different gels and blots
  • Attached lid – to protect experiments from dust or debris that can cause speckles on blot images
  • Save – minimize antibody and buffer usage by using appropriately sized containers
  • Multi-chamber trays – process 6 mini-blots simultaneously, each chamber requires as little as 3 mL of solution

 

AdvanWash washing solution
AdvanWash washing solution

Washing solutions optimized for use with WesternBright systems

  • Reliable – quality controlled wash buffer for reproducible results
  • Versatile – compatible with chemiluminescent and fluorescent Western blots
  • Convenient – save time using pre-made buffers; no more weighing, mixing, or adjusting pH

 

Western Blot Strip-It Buffer
Western Blot Strip-It Buffer

Stripping buffer for stripping antibodies from chemiluminescent Western blots

  • Conserve valuable samples - probe the same blot multiple times
  • Fast protocol - blot stripped and ready for re-probing in 25 minutes
  • Gentle protocol - antibodies removed without harsh reagents or elevated temperatures
  • Save time - no need to re-run gels and perform duplicate blots
  • Versatile - compatible with both nitrocellulose and PVDF membranes and with a variety of chemiluminescent detection reagents

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Blotting sponge pads
Blotting sponge pads

Transfer sponge pads for more reproducible Western blot transfers

  • Resist compression - maintain proper pressure in transfer sandwiches without adding extra blotting papers
  • Retain thickness - can be reused over multiple experiments
  • Convenient - two sizes (8 x 11 cm and 9 x 15 cm) compatible with a variety of transfer apparatuses
  • Standardized - uniform flat construction ensures reproducible and consistent transfers
  • Four pads per pack - a full set for common transfer apparatus
Blotting papers
Blotting papers

Pre-cut paper for electrophoretic transfers

  • Convenient – pre-cut; work with most mini gel transfer assemblies
  • Standardized – uniform thickness for reproducible transfer
  • Tested – perform consistently without artifacts or background
  • Available sizes include 7x9 cm and 8x10 cm
X-ray film cassette
X-ray film cassette

Light-tight film cassette for exposing blots

  •  Convenient - quickly load and unload with simple push-button latch
  • Light weight - quality light-weight aluminum construction
  • Proven performance - no light leaks even with overnight exposures