Stripping – the quick and dirty way to maximize the results from a single Western blot. It is tempting to use this technique to probe for an additional protein. But don't be seduced by this seemingly innocuous step. While there may be a time when ...

Western blot film – some is blue some is gray.  Some is even clear.  Then there are the boxes of pre-exposed film with black edges and corners that you only use when you have no other film left. Does it make a difference which film you use ...

It is tempting to take the film of your great experiment, pop it on the scanner and use densitometry to quantitiate your Western results. But what you calculate could be mis-leading and even down-right wrong. Here's why: ...

Back in the day (the good 'ole 1990's), things used to be so simple. When you wanted to develop your Western blot you would soak it in some chemiluminescent substrate then head to the dark room for some quality time by yourself with your Western ...