Some studies suggest that up to 30% of proteins are phosphorylated in eukaryotic cells.  Whoa. So chances are, if you study proteins, you are going to be examining protein phosphorylation at some point in your career.  But how do you know which ...

Blocking and washing can make or break a Western. Insufficient blocking or washing can lead to high background and a low signal-to-noise ratio. Excessive blotting or washing can mask antibody-antigen interactions or wash away antigen. Detection ...

Multiplexing is becoming "all the rage" these days. And why not? It's an ideal technique for simultaneously detecting multiple proteins or protein species. Want to have an in-lane normalization control? – Multiplex it Want to quantify ...

So you've decided to take the plunge and go for a total stain to normalize your Western blot. With some beginner's luck, it might turn out better than you have ever imagined.....or it might need some improvement. Try these helpful tips to improve ...