So you've decided to take the plunge and go for a total stain to normalize your Western blot. With some beginner's luck, it might turn out better than you have ever imagined.....or it might need some improvement. Try these helpful tips to improve ...
Posts Categorized: Protocols
Normalizing your Western blot is critical to obtaining accurate quantitative measurements. But it can be tricky. Perhaps you are discovering that housekeeping genes are not as neat and tidy as you would like? Then you might like to try total protein ...
Us scientists live up to our Frankenstein-producing alter egos when it comes to breaking down cells. We have almost as many ways to lyse cells as grant reviewers have of saying no. The question we ask today is: How do you grind your cells? ...
Photo courtesy of Steampunk Family the von Hedwigs Cocktails in the lab? Normally I wouldn't recommend it. But sometimes a good cocktail is what you need to get the job done right. When you lyse cells – either by adding detergents or by ...