You do a Western blot looking at a new protein and instead of one specific band you see a few bands.  One is close to the predicted molecular weight and is pretty distinct, but they others run more slowly indicating a greater molecular weight. ...

Larger proteins can be notoriously difficult to work with when Western blotting.  In particular, you can have a hard time achieving a good transfer efficiency.  In some ways,   large proteins are like teenagers:  they are gangly and awkward, ...

A few months ago, we launched one of our new products:  Visio, a protein loading buffer that allows you to watch your proteins as they migrate through the gel.  Compatible with downstream mass spectrometry, Visio contains a protein stain in the ...

Detecting phosphorylated proteins on a Western blot isn't really different from doing a regular Western blot.  But there are a few tricks that can help you get the perfect blot: ...