It's tradition – you perform a Western blot to observe the expression pattern of your protein of interest. Then, you also probe for housekeeping genes to prove that any changes you see are not due to loading inconsistencies nor a lack of protein ...
Posts Categorized: Fluorescent Westerns
Stripping – the quick and dirty way to maximize the results from a single Western blot. It is tempting to use this technique to probe for an additional protein. But don't be seduced by this seemingly innocuous step. While there may be a time when ...