Protein transfer from the gel to membrane is the most variable part of a Western blot. Even if you know the optimal buffer and transfer conditions, there are always bubbles, uneven protein transfer or other transfer artefacts, which you don’t ...

Setting up a new Western blot?  Thanks to increased assay sensitivities, readily available antibody-coupling products and improved conjugate reagents, you have a big decision to make:  Should you do a direct or an indirect Western blot? The ...

Ever thought about using directly-labeled primary antibodies for your assay?  You can buy them commercially, or you can label them yourself.  With new kits and technology, it's fairly easy to do. Here are some reasons why you might want to add ...

So you think your protein might be phosphorylated?  Maybe it runs as a fuzzy smear on the gel, slightly higher than predicted.  Or perhaps you think it is involved in a signaling cascade.  In any case, if you suspect phosphorylation, then you ...