View CartWestern blotting technical resources
Western blotting is a multi-step procedure which can require optimization of numerous factors. Advansta's guide to Protein Analysis includes advice for optimizing numerous variables including:
- Polyacrylamide percentage of gel for best protein separation
- Amount of protein loaded onto gel
- Selection of appropriate transfer membrane
- Dilution of primary and secondary antibodies
- Blocking conditions
- Detection methods
Download the Protein Analysis guide here:
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Protein analysis guide |
Advansta's Western blot detection reagents
For advice related to specific Advansta Western blotting detection systems, please refer to the user manuals for each product:
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WesternBright MCF user manual |
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WesternBright ECL user manual |
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WesternBright Quantum user manual |
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WesternBright Sirius user manual |
Or refer to the Advansta FAQ page to find answers to frequently asked questions.
General Western blotting troubleshooting
For a guide to trouble shooting the entire process, from sample preparation to electrophoresis to imaging, see our Western blotting troubleshooting page.
