Purification and clean-up products

Purification and clean-up products

Advansta offers a selection of products to make sample preparation more efficient. Quickly and easily clean up nucleotide and protein samples, so you can move on to data collection and analysis.

Remove protein from nucleic acids

RapidClean removes all protein from aqueous solutions of single- or double-stranded nucleic acids, providing a simple and non-toxic alternative to phenol-chloroform extraction procedures. The RapidClean resin completely removes protein in a 5-minute protocol that combines convenience, speed and a nucleic acid recovery rate in excess of 95%.

Figure 1. Extraction with RapidClean completely removes DNase I, Mung Bean Nuclease, and S1 nuclease activity. M12mp18 ssDNA (lane 1) is completely digested by 30 minute incubations at 37 C with DNase 1 (lane 2), Mung Bean Nuclease (lane 4) or S1 nuclease (lane 6), but no residual activity is observed if the reactions are first extracted twice with RapidClean (lanes 3, 5, and 7). Lane M: 1 kb DNA ladder.

SDS PAGE sample preparation

Afyon SDS-PAGE sample preparation kit provides a fast, efficient way to concentrate protein samples and remove buffer components that may interfere with electrophoresis. In less than 10 minutes, samples are ready to load on the gel. The Afyon protocol is easily scaled up so multiple samples can be processed in parallel, making it perfect for routine preparation of samples for SDS-PAGE and subsequent Western blot analysis.

Figure 2. Concentration of a protein sample using the Afyon kit. Figure 2a: Coomassie R-250 stained gel comparing initial A431 cell extract (lane 2), diluted cell extract (lane 3), and concentrated sample after the Afyon protocol (lane 4). Lane 1 is molecular weight marker. Figure 2b: profile of lanes on the stained gel, demonstrating that the banding patterns in initial and concentrated samples are identical.